ruthenium bipyridine triphenylphosphine caged gaba 39 rubi gaba  (Tocris)

Journal: Scientific Reports

Article Title: Tonic GABA A receptor currents in Cerebellar Purkinje cells of wild-type and DMD mdx mice

doi: 10.1038/s41598-025-31919-w

Figure Lengend Snippet: Tonic GABA currents in DMD mdx PCs are mediated by expression of δ-subunit containing GABA A receptors. ( A ) Representative current traces showing tonic GABA current measured in the presence of DS2 from WT or DMD mdx PC. ( B ) Average tonic GABA current measured in control solution or in the presence of DS2 in WT (black) and DMD mdx (blue) PCs. Values from individual cells are plotted as open circles. ( C ) Representative traces of currents evoked by photolytic uncaging of RuBi-GABA over the soma of WT and DMD mdx PCs using a range of laser powers (58–938 µW). ( D ) Average GABA current response plotted against uncaging laser power for WT (black) and DMD mdx (blue) PCs. Inset: Average EC 50 value for WT and DMD mdx derived from fitting the power-response curve for each cell with the Hill equation.

Article Snippet: In addition to the 10 μM NBQX and 10 μM CPP, 60 μM RuBi-GABA (Tocris) was added to the ACSF.

Techniques: Expressing, Control, Derivative Assay

Journal: bioRxiv

Article Title: Perineuronal Net and Inhibitory Synapse Remodeling on Striatal Fast-spiking Interneurons by Chronic Alcohol Exposure

doi: 10.1101/2025.07.08.663744

Figure Lengend Snippet: A ) Schematic of viral injection of virus expressing a cre-dependent Gephyrin-GFP intrabody into parvalbumin (PV)-cre mice. B ) Representative image of an FSI expressing gephyrin-GFP intrabody (green) colocalized to an immunofluorescent marker for vesicular GABA transporter (VGAT) puncta. VGAT positive puncta were masked onto the GFP signal and colocalized signals (white dots) were labeled with a dot function model to quantify GABAergic synapses (scale bar 10µm). (right) Representative fluorescent image of proximal neuronal branch from control and CIE treated mice depicting colocalized presynaptic VGAT (purple) puncta onto gephyrin (green)-positive postsynaptic elements (scale bar 1µm). CIE dramatically reduced the density of colocalized VGAT puncta onto somata ( C ), proximal dendrites ( D ), and distal dendrites ( E ) (N=8 mice per group, 4M 4F). F ) Schematic of whole-cell patch clamp recording from an FSI during photo-uncaging of RuBi-GABA. G ) Representative traces of photo-uncaged GABA currents from FSIs of control (left) and CIE (right) treated mice at increasing stimulus intensities (scale bar = 200pA, 100ms). H ) CIE did not impact photo-uncaged IPSCs compared to control treated mice (n=8 mice per group, 4M 4F). All data represented as mean + SEM. *P < .05, **p<.01. Portions of this figure were created with BioRender.com

Article Snippet: To photo-uncage GABA, a 2 ms pulse of blue light (470 nm) was delivered to an acute slice preparation bath perfused with 250 μM ruthenium-bipyridine-triphenylphosphine caged GABA (RuBi-GABA) (Tocris # 4709).

Techniques: Injection, Virus, Expressing, Marker, Labeling, Control, Patch Clamp

Journal: bioRxiv

Article Title: Perineuronal Net and Inhibitory Synapse Remodeling on Striatal Fast-spiking Interneurons by Chronic Alcohol Exposure

doi: 10.1101/2025.07.08.663744

Figure Lengend Snippet: A ) schematic of whole-cell patch recording from PNN enriched (PNN+) or PNN poor (PNN-) FSI identified by WFA stain after recordings. B ) PNN+ FSIs exhibited larger eIPSC amplitudes compared to undetected PNN-FSIs (N=11 PNN+ cells, 5M 6F; 6 PNN-cells, 3M 3F). Inset: representative traces from PNN+ (pink) and PNN+ (black) FSIs (scale bar 1nA, 100ms). C ) The presence of PNNs did not impact GABA release probability onto FSIs. D-F ) Spontaneous IPSC events (sIPCSs) recorded from FSIs (N=11 PNN+ cells, 5M 6F; 6 PNN-cells, 3M 3F). Inset: representative traces from PNN+(pink) and PNN-(black) FSIs (scale bar 100pA, 1s). D,E ) PNN+ FSIs exhibited a greater frequency of spontaneous IPSC events but ( F ) no change in IPSC amplitude compared to PNN-FSIs. G ) Schematic of unilateral microinjection of chondroitinase ABC (ChABC) into the dorsolateral striatum to enzymatically degrade PNNs. H ) Degrading PNNs with ChABC reduced eIPSC event amplitude onto FSIs but ( I ) did not impact GABA release probability (N=10 mice, 5M 5F; scale bar 1nA, 100ms). J,K ) Degrading PNNs with ChABC reduced the frequency of sIPSC events onto FSIs but ( L ) did not impact event amplitude (N=8 mice, 4M 4F; scale bar 100pA, 1s). M ) Schematic of viral injection of cre-dependent Gephyrin-GFP intrabody into PV-cre mice followed by ChABC. N) Degrading PNNs with ChABC did not reduce inhibitory synapses onto somata but ( O ) did reduce inhibitory synapse number on proximal dendrites (N= 13 mice, 6M 7F). P ) Schematic of whole-cell patch clamp recording during photo-uncaging of RuBi-GABA onto FSIs treated with ChABC. Q ) Representative traces of photo-uncaged GABA postsynaptic currents on control (left) and ChABC (right) treated FSIs at increasing stimulus intensity (scale bar = 200pA, 100ms). G ) There was no change in photo-uncaged IPSC event amplitude between control (black) and ChABC (pink) FSIs (N=8 mice per group, 4M 4F). All data represented as mean + SEM. *P < 0.05. ** P < 0.01. Portions of this figure were created with BioRender.com

Article Snippet: To photo-uncage GABA, a 2 ms pulse of blue light (470 nm) was delivered to an acute slice preparation bath perfused with 250 μM ruthenium-bipyridine-triphenylphosphine caged GABA (RuBi-GABA) (Tocris # 4709).

Techniques: Staining, Microinjection, Injection, Patch Clamp, Control